Discuss each of the following factors: If a higher voltage had been used, the DNA would have moved faster through the agar gel, and slower if the voltage was low.
Check out this interesting article using genetically altered goats to produce medications, now approved by the FDA! DNA fingerprinting lab Colin and Brianna Abstract The widespread use of electrophoresis has played an essential part in mapping the human genome.
The process of electrophoresis was developed in the mids and enhanced in the earlys. DNA fingerprinting is now used in many different areas of research including forensics and the study of bacterial epidemics.
This lab is meant to show the process of DNA fingerprinting through electrophoresis by simulating a crime scene using bacterial plasmids and restriction enzymes Eco-R1 and Pst1. The bacterial plasmids were used over human plasmids for simplicities sake.
The experiment was set up according to standard procedure for an agarose gel electrophoresis. The study showed how DNA fingerprinting helps find similarities in cleaving sites between different plasmid samples. The results were analyzed based on plasmid maps and the observed agarose gel.
The observed DNA fragments were compared to the expected fragments and some discrepancies were found. These could be due to errors in preparation method of the agarose gel. The benefits of DNA Dna fingerprinting gel electrophoresis restriction enzymes lab report stretch over all areas of science and help researchers discover new aspects of bacterial and human genomes.
Background The widespread use of electrophoresis has played an essential part in mapping the human genome Vesterberg. The discovery of the different parts of the genome has benefited many areas of investigative science.
DNA fingerprinting revolutionized forensics as a whole. It helps solve burglaries, murders, crime scenes, and even helps confirm paternity tests. At the Institute of Virology in Glasgow in the mids, Vin Thorne wanted to find a better way to classify the DNA forms that he was studying from purified polyomavirus.
Through his knowledge of frictional and electrical forces, he was able to use electrophoresis with agar to separate polyomavirus DNA fragments, but only after the segments expressed radioactive traits. Restriction enzymes are used to cut DNA into small sequences.
Eco-R1 and Pst1 are both commonly used enzymes that cut between the A and G bases. The method that is used today, however, came from a group of investigators working at Cold Spring Harbor Laboratory in the earlys. The investigators discovered that the fragments of DNA were more visible when the agar was dyed Sambrook and Russell.
Since the movement of the DNA fragments was unaffected by the dye the use of the dye became common practice and allowed researchers to insert photographs of the gel plates into computer databases. This simulated crime scene uses bacterial plasmids instead of human plasmids.
Bacterial plasmids are used instead of human plasmids because, in the educational laboratory setting, human plasmids, with 3 x base pairs, are too large. The bacterial plasmids used are significantly easier to work with because they are smaller, with the maximum number being 9, base pairs Hartl and Jones The electrophoresis was then set up according to Sambrook and Russell.
Results After electrophoresis was completed, the agarose gel showed the various DNA sequences from the crime scene along with plasmid sequences T1 through T5 from the simulated crime scene, followed by a ladder sequence.
The crime scene sequence had three DNA fragments close together, followed by another fragment much further down. The T2 showed similar spacing to the last two fragments, whereas the first fragment was spaced further apart, towards the top. T3 had a total of four fragments with three evenly spaced apart and one further down.
Unevenly spaced, there were three T4 fragments. In T5, there were four unevenly spaced fragments, starting half way down the gel. T5 was then followed by a ladder that started at the top end of the gel and contained three evenly spaced DNA fragments.
Discussion There are many different types of electrophoresis that are used for a cornucopia of research topics. The benefits of the larger fingerprints include bacteriophage typing and serotyping to determine the risk different bacteria are for causing an outbreak and also more accurate forensic DNA results Tenover et.
This is also the case in this experiment. In this experiment, each bacterial plasmid has specific fingerprints based on the plasmid maps Bio-Rad.
However, the observed results differed slightly from what was expected. The different bacterial plasmids in the agar were missing some expected fragments. This shows that the restriction enzymes did not cleave the plasmid DNA at the proper places.
For T1 and T2 the expected result was to have 4 DNA fragments but the observed results only showed 3. For T4, the expected result was to have 4 DNA fragments but only 3 were observed and T5 had the largest discrepancy with the expected results being 8 and the observed result being 4. However, the hypothesis was supported because the observed results from the CS and T3 plasmids matched and had the expected number of DNA fragments.The materials needed for this lab are the following: an electrophoresis chamber, an agarose gel, lambda DNA digested with endonucleases, tracking dye, micropipette .
ABSTRACT. Gas production from microbial deterioration in vacuum-packs of chilled meat leads to pack distension, which is commonly referred as blown pack.
Restriction Digestion and Analysis of Lambda DNA Kit Instruction Manual Catalog #EDU DNA fingerprinting, and forensic DNA analysis. your students must first identify the gene by its size using agarose gel electrophoresis.
Restriction Enzymes The ability to cut and paste, or cleave and ligate, a functional piece of DNA. The history of the polymerase chain reaction (PCR) has variously been described as a classic "Eureka!" moment, or as an example of cooperative teamwork between disparate researchers.
Following is a list of events before, during, and after its development. This lab is meant to show the process of DNA fingerprinting through electrophoresis by simulating a crime scene using bacterial plasmids and restriction enzymes Eco-R1 and Pst1.
The bacterial plasmids were used over human plasmids for simplicities sake. DNA profiling (also called DNA fingerprinting) is the process of determining an individual's DNA characteristics, which are as unique as urbanagricultureinitiative.com analysis intended to identify a species, rather than an individual, is called DNA barcoding..
DNA profiling is a forensic technique in criminal investigations, comparing criminal suspects' profiles to DNA evidence so as to assess the.