Effect of temperature enzyme catalyse hydrogen peroxide measuring oxygen

As with the chemical antioxidants, cells are protected against oxidative stress by an interacting network of antioxidant enzymes. This detoxification pathway is the result of multiple enzymes, with superoxide dismutases catalysing the first step and then catalases and various peroxidases removing hydrogen peroxide. As with antioxidant metabolites, the contributions of these enzymes to antioxidant defenses can be hard to separate from one another, but the generation of transgenic mice lacking just one antioxidant enzyme can be informative. Here, its cofactor is oxidised by one molecule of hydrogen peroxide and then regenerated by transferring the bound oxygen to a second molecule of substrate.

Effect of temperature enzyme catalyse hydrogen peroxide measuring oxygen

For this experiment, the independent variable is the temperature of the potato and hydrogen peroxide. The dependent variable is the reaction time in the test tube.

This is determined by measuring the time it takes for the paper to float to the top of the test tube. The constants control variables are the amount of hydrogen peroxide used, amount of potato used and the size of the filter paper.

The potato is cut into small pieces and, together with some water, blended until it becomes soft.

Effect of temperature enzyme catalyse hydrogen peroxide measuring oxygen

The filter paper is cut into 8 pieces of small squares 5mm by 5mm each, and each piece is then dipped in the blended potato using tweezers. The test tubes are also marked with a line 8mm from the bottom of the tube. The hydrogen peroxide solution is poured into the 8 test tubes up to the level of the line.

The 8 beakers are filled with water up to 8cm high.

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Place the test tubes marked 1 to 8 into the beakers marked 1 to 8 accordingly for 5 minutes, to enable the hydrogen peroxide to stabilize at the same temperature. Put the filter paper dipped with the blended potato into the test tubes one at a time. Start the stopwatch and check the time it takes for the paper to reach the surface.

Effect of temperature enzyme catalyse hydrogen peroxide measuring oxygen

Record the measurements in the table below.This enzyme helps to convert hydrogen peroxide into oxygen and water. Chemical activities that happen within the cell produces hydrogen peroxide, which is poisonous and can kill the organism. The presence of the enzyme catalase in the cell helps to quickly convert this toxic substrate into safer products of water and oxygen.

Effect of Temperature (C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10, 37, 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that.

A paradox in metabolism is that, while the vast majority of complex life on Earth requires oxygen for its existence, oxygen is a highly reactive molecule that damages living organisms by producing reactive oxygen species.

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Consequently, organisms contain a complex network of antioxidant metabolites and enzymes that work together to . • Measure the production of oxygen gas as hydrogen peroxide is destroyed by the enzyme catalase or peroxidase at various temperatures. • Measure and compare the initial rates of reaction for the enzyme at each temperature.

Examination of the nutritional deficiencies that affect Chronic Fatigue Sufferers: mineral, vitamin and fatty acid.

Aim: The aim of the Assessment Task 1 is to investigate the effect of 1)temperature, 2)pH and 3)substrate concentration on the action of enzyme such as catalase on hydrogen peroxide.

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