Hptlc method for determination of edaravone

Find articles by Archana P. This article has been cited by other articles in PMC. Abstract Mucuna pruriens Linn. L-DOPA extracted from seeds of Mucuna is a constituent of more than indigenous drug formulations and is more effective as drug than the synthetic counterpart.

Hptlc method for determination of edaravone

Sunitha 1 and K. A simple, precise, specific and accurate high-performance thin-layer chromatographic method has been developed for the simultaneous determination of Emtricitabine EMB and Tenofovir TEN in pharmaceutical dosage form.


HPTLC separation of the two drugs followed by densitometric measurement was carried out in the absorbance mode at nm. The drugs were resolved satisfactorily with Rf values of 0.

The method was validated for precision, robustness, specificity and accuracy. Emtricitabine and Tenofovir Fig. Emtricitabine is chemically 4-Amino - 5 - fluoro - 1 - [2- hydroxymethyl -1, 3-oxathiolanyl]-pyrimidinone 1, 2. It is classified as a nucleoside reverse transcriptase inhibitor.

Chemically Tenofovir is 1- 6-Aminopurinyl -prapanyl-oxymethylphosphonic acid 3, 4. It is a nucleotide analogue reverse transcriptase inhibitor.

HPTLC method is cost effective, rapid and less time consuming. The proposed method is validated as per ICH guidelines Instrumentation and Chromatographic Conditions: A constant application rate of 0.


The mobile phase consisted of toluene: The length of chromatogram run was 80mm and run time was 20min. The source of radiation used was a deuterium lamp emitting a continuous UV spectrum between and nm.

All determinations were performed at ambient temperature with a detection wavelength of nm.

Hptlc method for determination of edaravone

Concentrations of the compound chromatographed were determined from the intensity of the diffused light. Evaluation was by peak areas with linear regression.

For calibration, EMB 10 mg and TEN 10mg were weighed separately, dissolved in methanol in 10mL volumetric flasks and volume adjusted to give standard stock solution of ppm.


The plate was then developed using the previously described mobile phase. The peak areas were plotted against the corresponding concentrations to obtain the calibration graphs.

Linear calibration curves were generated using least-squares linear-regression analysis. Analysis of Commercial Formulation: To determine the content of EMB and TEN simultaneously in pharmaceutical dosage form, twenty tablets were weighed accurately and average weight calculated.

Quantity equivalent to mg of EMB and mg of TEN was taken in ml volumetric flask and dissolved in 25ml of methanol and the final volume was made up with the same solvent. The solution was filtered through 0. The plate was developed in the previously described chromatographic conditions and the peak area of the spots was measured at nm and the concentrations in the samples were determined using multilevel calibration developed on the same plate under the same conditions using linear regression equation.Rapid, accurate and precise method for identification and quantitation of Olmesartan medoxomil (OLM) was developed by HPTLC-densitometry and validated.

The Camag HPTLC system, employed with software winCATS (ver) was used for proposed analytical work. that the method is simple, specific, selective and reliable for simultaneous quantitative determination of dexamethasone phosphate and chloramphenicol in pharmaceutical formulations. The developed HPTLC method.

A rapid, accurate, specific, repeatable and robust HPTLC method for the determination of lycorine in different Amaryllidaceae plant extracts is presented in this work. Validated HPTLC Method for the Determination of two No vel Steroids in Bulk and Pressurized Metered-Dose Preparations Int.

J. Appl. Sci. Eng., 9, . Comparative determination of miconazole, nystatin, hydrocortisone and neomycin by HPTLC/HPLC-DAD Development and validation of HPTLC method for the estimation of clotrimazole in bulk drug and tablet formulation.

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